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collagen type i  (Bioss)
94
Bioss collagen type i
Collagen Type I, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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col1a1 antibody  (Proteintech)
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Proteintech col1a1 antibody
1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Col1a1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human collagen type i alpha 1 col1a1 elisa kit  (ABclonal Biotechnology)
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1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Human Collagen Type I Alpha 1 Col1a1 Elisa Kit, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1a1/COL1A1+Rabbit+pAb/10__1016_slash_j__bioadv__2026__215236-257-1-10
Average 98 stars, based on 1 article reviews
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anti col1a1  (ABclonal Biotechnology)
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ABclonal Biotechnology anti col1a1
1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Anti Col1a1, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1a1/COL1A1+Rabbit+pAb/pm42816544-103-23-26
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col1a1  (SouthernBiotech)
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SouthernBiotech col1a1
1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Col1a1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1a1/Goat+Anti-Type+I+Collagen-UNLB/pm42806145-1039-0-2
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anti col1a1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti col1a1
1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Anti Col1a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1a1/COL1A1+Mouse+mAb/pmc13655729-114-10-11
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col1a1  (Cell Signaling Technology Inc)
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1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Col1a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/col1a1/COL1A1+Rabbit+mAb/10__1038_slash_s41598___026___70412___w-294-77-79
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anti acta2  (ABclonal Biotechnology)
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1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
Anti Acta2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, <t>Col1a2,</t> TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and <t>COL1A1</t> (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.
72026s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti col1a1 primary antibody  (ABclonal Biotechnology)
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All the primers listed in this study.
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Image Search Results


1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, Col1a2, TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and COL1A1 (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Renal Tubular Epithelial Cells Senescence Induced by 1‐Aminopyrene Activates Fibroblast via BRD4 Driven Senescence‐Associated Secretory Phenotype Signaling

doi: 10.1002/jbt.71124

Figure Lengend Snippet: 1 ‐ AP induces tubular epithelial senescence and promotes paracrine fibroblast activation via a SASP‐mediated mechanism. (A) Representative images and quantification of SA‐β‐gal positive RTECs following exposure to 1‐AP after 72 h. Scale bars, 5 µm. (B) DAPI‐stained nuclei (blue) and quantification showing a significant reduction in cell number following exposure to 1‐AP after 72 h. Scale bars, 2 µm. (C) Representative images and quantification of immunofluorescence analysis of Ki67 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (D) RT‐qPCR analysis of p16, p21, and p53 mRNA expression showing upregulation of senescence‐associated cell cycle regulators in RTECs following exposure to 1‐AP after 48 h. (E) Representative images and quantification of immunofluorescence analysis of p21 in RTECs following 1‐AP treatment after 72 h. Scale bars, 5 µm. (F) Heatmap for RT‐qPCR analysis of senescence‐associated secretory phenotype (SASP) factors (IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, and CXCL1) in RTECs following 1‐AP treatment after 48 h. (G) Schematic illustration of the experimental design showing 1‐AP induced senescence in RTECs and paracrine activation of renal fibroblasts via conditioned media may leads to TIF or renal fibrosis. (H) Heatmap for RT‐qPCR analysis of fibrotic markers ( α‐SMA, POSTN, Col1a2, TIMP1 , and CTGF ) in NRK52F renal fibroblasts exposed to conditioned media from 1‐AP‐treated TECs for 48 h. (I–K) Representative images and quantification of immunofluorescence analysis of α‐SMA (I), POSTN (J), and COL1A1 (K) in NRK52F fibroblasts, demonstrating significant fibroblast activation and extracellular matrix induction following exposure to TEC‐derived conditioned media after 48 h. Scale bars, 20 µm. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Article Snippet: Primary antibodies for AhR antibody (182705‐10 G, Meck), Phospho‐Histone H2A.X (Ser139) Antibody (20E3, cell signaling), BRD4 antibody (ER1901‐02, Thermo‐Fischer), Ki‐67 antibody (27309‐1‐AP, proteintech), p21 (2947, cell signaling), α‐SMA antibody (14395‐1‐AP, proteintech), periostin (19899‐1‐AP, proteintech), COL1A1 antibody (55131‐1‐AP, proteintech), Histone H3K9/14ac (Acetyl H3K9/14) Antibody (A‐4021‐025, epigentek), Histone H3K27me3 (H3K27 Trimethyl) Antibody (A‐4039‐025, epigentek), Histone H3K9me3 (H3K9 Trimethyl) Antibody (A‐4036‐025, epigentek), IL‐6 (21865‐1‐AP, proteintech), TGF‐beta1 (21898‐1‐AP, proteintech) and Secondary antibody anti‐Mouse Alexa‐Fluro 555 ( A31570 , Thermo‐Fischer) and anti‐Rabbit Alexa‐Fluro 488 (A11008, Thermo‐Fischer), TUNEL Assay Kit (25879, cell signaling) and Senescence β‐Galactosidase (SA‐β‐gal) Staining Kit (9860, cell signaling) were purchased.

Techniques: Activation Assay, Staining, Immunofluorescence, Analysis, Quantitative RT-PCR, Expressing, Derivative Assay, Two Tailed Test

AhR signaling is activated in RTECs by 1‐AP but is insufficient for SASP‐mediated fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of AHR expression in RTECs following 1‐AP treatment ± AhR inhibitor (10 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of AhR target genes ( CYP1A1, CYP1A2 ) and senescence markers ( p16, p21, p53 ) following 1‐AP treatment ± AhR inhibitor after 48 h. (C) Heatmap for RT‐qPCR analysis of SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) following 1‐AP treatment ± AhR inhibitor after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± AhR inhibitor to NRK‐52F fibroblasts after 48 h showing (D) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (E–G) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Renal Tubular Epithelial Cells Senescence Induced by 1‐Aminopyrene Activates Fibroblast via BRD4 Driven Senescence‐Associated Secretory Phenotype Signaling

doi: 10.1002/jbt.71124

Figure Lengend Snippet: AhR signaling is activated in RTECs by 1‐AP but is insufficient for SASP‐mediated fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of AHR expression in RTECs following 1‐AP treatment ± AhR inhibitor (10 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of AhR target genes ( CYP1A1, CYP1A2 ) and senescence markers ( p16, p21, p53 ) following 1‐AP treatment ± AhR inhibitor after 48 h. (C) Heatmap for RT‐qPCR analysis of SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) following 1‐AP treatment ± AhR inhibitor after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± AhR inhibitor to NRK‐52F fibroblasts after 48 h showing (D) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (E–G) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Article Snippet: Primary antibodies for AhR antibody (182705‐10 G, Meck), Phospho‐Histone H2A.X (Ser139) Antibody (20E3, cell signaling), BRD4 antibody (ER1901‐02, Thermo‐Fischer), Ki‐67 antibody (27309‐1‐AP, proteintech), p21 (2947, cell signaling), α‐SMA antibody (14395‐1‐AP, proteintech), periostin (19899‐1‐AP, proteintech), COL1A1 antibody (55131‐1‐AP, proteintech), Histone H3K9/14ac (Acetyl H3K9/14) Antibody (A‐4021‐025, epigentek), Histone H3K27me3 (H3K27 Trimethyl) Antibody (A‐4039‐025, epigentek), Histone H3K9me3 (H3K9 Trimethyl) Antibody (A‐4036‐025, epigentek), IL‐6 (21865‐1‐AP, proteintech), TGF‐beta1 (21898‐1‐AP, proteintech) and Secondary antibody anti‐Mouse Alexa‐Fluro 555 ( A31570 , Thermo‐Fischer) and anti‐Rabbit Alexa‐Fluro 488 (A11008, Thermo‐Fischer), TUNEL Assay Kit (25879, cell signaling) and Senescence β‐Galactosidase (SA‐β‐gal) Staining Kit (9860, cell signaling) were purchased.

Techniques: Activation Assay, Immunofluorescence, Analysis, Expressing, Quantitative RT-PCR, Two Tailed Test

BRD4 recruitment in RTECs sustains SASP transcription and paracrine fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of BRD4 expression in RTECs following 1‐AP treatment ± JQ1 (0.5 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of senescence regulators ( p16, p21, p53 ) and SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) in RTECs following 1‐AP treatment ± JQ1 after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± JQ1 to NRK‐52F fibroblasts after 48 h showing (C) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (D–F) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Renal Tubular Epithelial Cells Senescence Induced by 1‐Aminopyrene Activates Fibroblast via BRD4 Driven Senescence‐Associated Secretory Phenotype Signaling

doi: 10.1002/jbt.71124

Figure Lengend Snippet: BRD4 recruitment in RTECs sustains SASP transcription and paracrine fibroblast activation. (A) Representative images and quantification of immunofluorescence analysis of BRD4 expression in RTECs following 1‐AP treatment ± JQ1 (0.5 μM) after 48 h. Scale bars, 20 µm. (B) Heatmap for RT‐qPCR analysis of senescence regulators ( p16, p21, p53 ) and SASP genes ( IL‐1β, IL‐6, IL‐8, MCP‐1, TGF‐β1, CXCL1 ) in RTECs following 1‐AP treatment ± JQ1 after 48 h. Conditioned media (CM) transfer from NRK‐52E/RTECs treated with 1‐AP ± JQ1 to NRK‐52F fibroblasts after 48 h showing (C) Heatmap for RT‐qPCR analysis of fibroblast activation and extracellular matrix genes ( α‐SMA, POSTN, Col1a2, TIMP‐1, CTGF ), (D–F) Quantification of immunofluorescence analysis of α‐SMA, POSTN and COL1A1 in NRK52F fibroblasts. Data are presented as mean ± SD, n = 3. Statistical analysis was performed using two‐tailed unpaired Student's t‐test. Exact p ‐values are indicated in the graphs; ns, not significant. DAPI used as nuclear counterstain.

Article Snippet: Primary antibodies for AhR antibody (182705‐10 G, Meck), Phospho‐Histone H2A.X (Ser139) Antibody (20E3, cell signaling), BRD4 antibody (ER1901‐02, Thermo‐Fischer), Ki‐67 antibody (27309‐1‐AP, proteintech), p21 (2947, cell signaling), α‐SMA antibody (14395‐1‐AP, proteintech), periostin (19899‐1‐AP, proteintech), COL1A1 antibody (55131‐1‐AP, proteintech), Histone H3K9/14ac (Acetyl H3K9/14) Antibody (A‐4021‐025, epigentek), Histone H3K27me3 (H3K27 Trimethyl) Antibody (A‐4039‐025, epigentek), Histone H3K9me3 (H3K9 Trimethyl) Antibody (A‐4036‐025, epigentek), IL‐6 (21865‐1‐AP, proteintech), TGF‐beta1 (21898‐1‐AP, proteintech) and Secondary antibody anti‐Mouse Alexa‐Fluro 555 ( A31570 , Thermo‐Fischer) and anti‐Rabbit Alexa‐Fluro 488 (A11008, Thermo‐Fischer), TUNEL Assay Kit (25879, cell signaling) and Senescence β‐Galactosidase (SA‐β‐gal) Staining Kit (9860, cell signaling) were purchased.

Techniques: Activation Assay, Immunofluorescence, Analysis, Expressing, Quantitative RT-PCR, Two Tailed Test

All the primers listed in this study.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: All the primers listed in this study.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Sequencing

Quantitative analysis of collagen abundance in the calipash and muscle. ( A , B ) Representative Masson’s trichrome-stained sections utilized for collagen quantification in calipash ( A ) and muscle ( B ). ( A1 – A3 ) The first/second/third microscopic field in calipash; ( B1 – B3 ) The first/second/third microscopic field in muscle. The microscopic images are representative fields from the same individual and were used for visualization only. ( C ) Quantitative comparison of the collagen area fraction between the calipash and muscle tissues. Data are presented as mean ± SD ( n = 3) calculated from three independent microscopic fields. * p < 0.05 indicates significant differences.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Quantitative analysis of collagen abundance in the calipash and muscle. ( A , B ) Representative Masson’s trichrome-stained sections utilized for collagen quantification in calipash ( A ) and muscle ( B ). ( A1 – A3 ) The first/second/third microscopic field in calipash; ( B1 – B3 ) The first/second/third microscopic field in muscle. The microscopic images are representative fields from the same individual and were used for visualization only. ( C ) Quantitative comparison of the collagen area fraction between the calipash and muscle tissues. Data are presented as mean ± SD ( n = 3) calculated from three independent microscopic fields. * p < 0.05 indicates significant differences.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Analysis, Staining, Comparison

Comparative Picro Sirius Red staining of collagen subtype composition and spatial organization. ( A1 – A4 ) Representative brightfield microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( A1 ), 100 μm ( A2 ), 50 μm ( A3 ) and 20 μm ( A4 ). ( B1 – B4 ) Representative polarized light microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( B1 ), 100 μm ( B2 ), 50 μm ( B3 ) and 20 μm ( B4 ). ( C1 – C4 ) Representative brightfield microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( C1 ), 100 μm ( C2 ), 50 μm ( C3 ) and 20 μm ( C4 ). ( D1 – D4 ) Representative polarized light microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( D1 ), 100 μm ( D2 ), 50 μm ( D3 ) and 20 μm ( D4 ). Yellow-orange birefringence indicates densely packed type I collagen, while green birefringence is indicative of type III collagen-rich regions. Scale bars are as indicated in the respective images.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Comparative Picro Sirius Red staining of collagen subtype composition and spatial organization. ( A1 – A4 ) Representative brightfield microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( A1 ), 100 μm ( A2 ), 50 μm ( A3 ) and 20 μm ( A4 ). ( B1 – B4 ) Representative polarized light microscopy images of the calipash acquired at increasing magnification. Scale bars: 200 μm ( B1 ), 100 μm ( B2 ), 50 μm ( B3 ) and 20 μm ( B4 ). ( C1 – C4 ) Representative brightfield microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( C1 ), 100 μm ( C2 ), 50 μm ( C3 ) and 20 μm ( C4 ). ( D1 – D4 ) Representative polarized light microscopy images of muscle acquired at increasing magnification. Scale bars: 200 μm ( D1 ), 100 μm ( D2 ), 50 μm ( D3 ) and 20 μm ( D4 ). Yellow-orange birefringence indicates densely packed type I collagen, while green birefringence is indicative of type III collagen-rich regions. Scale bars are as indicated in the respective images.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Staining, Brightfield, Microscopy, Light Microscopy

Comparative immunofluorescence localization of COL1A1 in calipash ( A1 – A3 ) and muscle ( B1 – B3 ) tissues. ( A1 ) Representative immunofluorescence image showing the spatial distribution of COL1A1 (green) in calipash at low magnification; scale bar, 200 μm. ( A2 ) Higher-magnification image of a representative field of view in calipash; scale bar, 50 μm. ( A3 ) Higher-magnification image of a second, independent field of view in calipash; scale bar, 50 μm. ( B1 ) Spatial distribution of COL1A1 (red) in muscle tissue at low magnification; scale bar, 200 μm. ( B2 ) Higher-magnification image of a representative field of view in muscle; scale bar, 50 μm. ( B3 ) Higher-magnification image of a second, independent field of view in muscle; scale bar, 50 μm. Nuclei were counterstained with DAPI (blue). Scale bars are as indicated in the respective images.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Comparative immunofluorescence localization of COL1A1 in calipash ( A1 – A3 ) and muscle ( B1 – B3 ) tissues. ( A1 ) Representative immunofluorescence image showing the spatial distribution of COL1A1 (green) in calipash at low magnification; scale bar, 200 μm. ( A2 ) Higher-magnification image of a representative field of view in calipash; scale bar, 50 μm. ( A3 ) Higher-magnification image of a second, independent field of view in calipash; scale bar, 50 μm. ( B1 ) Spatial distribution of COL1A1 (red) in muscle tissue at low magnification; scale bar, 200 μm. ( B2 ) Higher-magnification image of a representative field of view in muscle; scale bar, 50 μm. ( B3 ) Higher-magnification image of a second, independent field of view in muscle; scale bar, 50 μm. Nuclei were counterstained with DAPI (blue). Scale bars are as indicated in the respective images.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Immunofluorescence, Tissue

Collagen gene expression profiling, transcriptional validation, and protein-level confirmation in calipash and muscle of P. sinensis . ( A ) Volcano plot of collagen-related DEGs. Each point represents a gene, with significantly upregulated (green) and downregulated (orange) genes in calipash defined by |log 2 fold change| ≥ 1 and FDR < 0.05. The x -axis represents log 2 fold change, and the y -axis represents log 10 adjusted p value. Collagen-related genes are highlighted, including col1a1 , col1a2 , col3a1 , col6a1 , col6a2 , col4a6 , col4a5 , col5a3 , col7a1 , col12a1 , col16a1 , and col17a1 . ( B ) Targeted qPCR validation of col1a1 and col1a2 expression in calipash and muscle. Data are presented as mean ± SD ( n = 3). Asterisks indicate significant differences between calipash and muscle ( p < 0.05). ( C ) Western blot analysis of COL1A1 protein in calipash and muscle. β-actin was used as the loading control.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: Collagen gene expression profiling, transcriptional validation, and protein-level confirmation in calipash and muscle of P. sinensis . ( A ) Volcano plot of collagen-related DEGs. Each point represents a gene, with significantly upregulated (green) and downregulated (orange) genes in calipash defined by |log 2 fold change| ≥ 1 and FDR < 0.05. The x -axis represents log 2 fold change, and the y -axis represents log 10 adjusted p value. Collagen-related genes are highlighted, including col1a1 , col1a2 , col3a1 , col6a1 , col6a2 , col4a6 , col4a5 , col5a3 , col7a1 , col12a1 , col16a1 , and col17a1 . ( B ) Targeted qPCR validation of col1a1 and col1a2 expression in calipash and muscle. Data are presented as mean ± SD ( n = 3). Asterisks indicate significant differences between calipash and muscle ( p < 0.05). ( C ) Western blot analysis of COL1A1 protein in calipash and muscle. β-actin was used as the loading control.

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: Gene Expression, Profiling, Biomarker Discovery, Expressing, Western Blot, Analysis, Control

DEGs related to collagen in the muscle and calipash of P. sinensis .

Journal: Animals : an Open Access Journal from MDPI

Article Title: Integrated Histological and Transcriptomic Analyses Reveal Distinct Structural and Molecular Features of the Calipash and Muscle in the Chinese Soft-Shelled Turtle ( Pelodiscus sinensis )

doi: 10.3390/ani16182960

Figure Lengend Snippet: DEGs related to collagen in the muscle and calipash of P. sinensis .

Article Snippet: After blocking with 10% goat serum (Boster Biological Technology Co., Ltd., Wuhan, China) for 30 min, the sections were incubated with a rabbit anti-COL1A1 primary antibody (ABclonal Biotechnology Co., Ltd., Wuhan, China; 1:200 dilution) overnight at 4 °C.

Techniques: